Research Profiles: Multipurpose nanopore sensors

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Nanopore sensors for nucleic acid analysis.

Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resol...

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Lipid bilayer coated Al(2)O(3) nanopore sensors: towards a hybrid biological solid-state nanopore.

Solid-state nanopore sensors are highly versatile platforms for the rapid, label-free electrical detection and analysis of single molecules, applicable to next generation DNA sequencing. The versatility of this technology allows for both large scale device integration and interfacing with biological systems. Here we report on the development of a hybrid biological solid-state nanopore platform ...

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Single-molecule protein identification by sub-nanopore sensors

Recent advances in top-down mass spectrometry enabled identification of intact proteins, but this technology still faces challenges. For example, top-down mass spectrometry suffers from a lack of sensitivity since the ion counts for a single fragmentation event are often low. In contrast, nanopore technology is exquisitely sensitive to single intact molecules, but it has only been successfully ...

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Highly Sensitive, Mechanically Stable Nanopore Sensors for DNA Analysis.

Understanding the biophysics governing single-molecule transport through solid-state nanopores is of fundamental importance in working toward the goal of DNA detection and genome sequencing using nanopore-based sensors. Even with significant advances in semiconductor fabrication technologies, the state-of-the-art in nanopore technology still falls well short of mimicking the elegance and functi...

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Silicon-On-Insulator Nanopore Arrays for Ion Channel Sensors

Ion channels are transmembrane proteins that facilitate the diffusion of ions and small molecules across the phospholipid bilayer membrane of cells. These proteins possess the capability to open and close due to various stimuli, including: voltage, temperature, pressure and ligand binding events. This ability defines the proteins as very small natural sensing elements [1]. The study of ion chan...

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ژورنال

عنوان ژورنال: Analytical Chemistry

سال: 2001

ISSN: 0003-2700,1520-6882

DOI: 10.1021/ac0124639